Factor V Leiden & Prothrombin gene analysis (G20210A)

Pathology category

Haematology

Code

FVL

Referral code

Request form

Standard

Test information

Only patients with reduced APC resistance or positive family history will be tested

Please Note: paediatric samples (<16 yrs old) will be referred to Special Coagulation Department, Birmingham Children’s Hospital, Steelehouse Lane, B4 6NH Telephone: 0121 333 9999

When FVL is requested on ICE it will automatically request Prothrombin gene analysis (PTV) in the laboratory and does not need to be requested separately

Guidelines

Pre-analytical

Sample transport should be at 4-25C and reach the laboratory ASAP

Please note: the samples must arrive within 24 hours of collection otherwise they will be rejected by the laboratory

Reference range

Diagnostic

Tube type

Tube type info

2.7ml Blue top citrate

1.8 ml paediatric citrate

Special requirements

Patients on heparin therapy and recently transfused patients may have blood specimens that potentially interfere with the PCR results and lead to invalid or erroneous results

Minimum volume

1 tube minimum 90% full (to the thick black line on the bottle) – essential to ensure 1:9 ratio of anticoagulant:blood

Assay frequency

As required – non routine

TAT Inpatient (urgent)

NA

TAT Inpatient

Discuss with lab

TAT GP/Outpatient

Discuss with lab

Referral lab

Local